Dental caries is progressive damage to the teeth caused by microbial organisms and is also most common disease affecting 80% of the population. Now a days, Restorative dentistry has moved away from a “drill and fill” philosophy to a minimally invasive approach. Several decades ago, increased Auto fluorescence has been found in the carious dentin which was supposed to be emitted by bacterial metabolites, and developed a novel caries excavation device based on fluorescence properties of dentin. Fluorescence is a visible light system that offers the opportunity to differentiate infected dentin and sound dentin using two forms of fluorescence Red and Green.The improved removal of infected dentin achieved using this new technique may be appealing to the clinician. Because it reduces the clinical working time.This also puts the dentist in a position to make an “informed decision” for the first time regarding the removal of carious dentin near the pulp. Key Words: Fluorescence, Dental Caries, Infected Dentin, Caries Excavation