Background: Dengue fever is an emerging disease occurring in tropical and subtropical areas. The present study was conducted to assess the knowledge, attitude and practices regarding transmission and prevention of dengue fever and to determine the association between knowledge and practice. Materials & Methods: The present study was conducted on 366 subjects of both genders. A pretested, semi-structured questionnaire was used as the data collection tool. It contains questions regarding knowledge about Dengue fever and the mode of disease transmission and modalities of prevention and treatment of the same. The attitude questions pertained to issues such as, disease carriers as source of infection and usage of preventive measure and disease curability. Results: out of 366 patients, males were 181 and females were 185. Age group 18-30 years had 217. 31-45 years had 103 and 46-65 years had 46 patients. The difference was significant (P< 0.05). Maximum number of subjects (356) heard about dengue fever from colleagues. 366 subjects were aware of transmission of dengue from mosquito. 263 subjects knew that dengue can be prevented with coils. 137 subjects knew that dengue spreads through mosquito larvae. Conclusion: Mosquito coils were the most popular preventive measure employed. Presence of the vectors was also noted in a third of the households.