Background: Iron is vital for all living organisms because it is essential for multiple metabolic processes, including DNA synthesis and oxygen and electron transport. Iron deficiency is a main nutritional deficiency disorder affecting large fractions of the world population and is a common cause of anemia. Iron deficiency anemia (IDA) is characterized by a defect in hemoglobin synthesis, resulting in red blood cells that are abnormally small (microcytic) and contain a decreased amount of hemoglobin (hypochromic). Aim of the study: To evaluate the lymphocytic count in pre-menopausal women with iron deficiency anemia. Materials and methods: The study was conducted in the Department of General Pathology of the medical institution. For the study, we selected 50 pre-menopausal women between the age group of 18-35 years. It was made sure that the subjects selected for the study are anemic having hemoglobin less than 10 g/dL. For the control group, 50 pre-menopausal women with normal hemoglobin level were included. The patients with thalassemia, leukemia or any other chronic and autoimmune disease were excluded from the study. Laboratory evaluation of each subject was done. Results: The mean age of the patients in study group was 26.12 years and in control group was 25.22 years. There were 50 subjects in each group. The mean CD3+, CD4+, CD8+, and CD19+ lymphocyte counts were 1.56, 0.79, 0.55, and 0.39 X109/L, respectively, in study group, and 1.89, 0.49, 0.71, and 0.29 X 109/L, respectively, for the control group. The absolute T lymphocytes (CD3+) and subpopulations (CD4+, CD8+) in the iron-deficient group were significantly lower than in the control group. On comparing the results, we observed that CD3+ lymphocyte count and CD3+/CD4+ lymphocyte count was statistically significant. Conclusion: The pre-menopausal women with iron deficiency anemia have significant change in the total lymphocyte count. Due to decreased lymphocyte count in pre-menopausal women with IDA, these patients may be more prone to infection. Keywords: Anemia, Iron deficiency, Hemoglobin