Background: One of the leading causes of death across the world is Acute myocardial infarction (AMI). Alteration in serum potassium levels is known to occur in patients with AMI. Hence; we planned the present study to assess the serum potassium levels in AMI patients. Materials & methods: The present research was conducted on 30 AMI patients and 30 healthy controls and it involved assessment and comparison of serum potassium levels in all the subjects. Blood samples were taken and sent to the laboratory for the evaluation of serum potassium levels. Auto-analyser was used for assessment of serum potassium levels. All the results were recorded and analysed by SPSS software. Results: Mean serum potassium levels of the subjects of the AMI group was 4.5 mEq/L while mean serum potassium levels of the subjects of the control group was 4.1 mEq/L respectively. Non- significant results were obtained while comparing the mean serum potassium levels among subjects of the control group and the AMI group. Conclusion: Electrolyte disturbance might contribute towards the pathophysiology of AMI. Key words: Acute myocardial infarction, Potassium