Background: Urinary tract infection (UTI) means growth of microorganism in urinary tract. There is an increased risk of UTI in patients with spinal cord injury (SCI) that accounts an overall incidence nearly 2.5 cases per year in India. The present study was aimed to estimate the prevalence of UTI in patients with SCI and to assess knowledge and practices regarding its prevention among them and their caregivers in a Tertiary care centre.Materials and Methods: A descriptive approach was used. Target population was patients with SCI and their caregivers. Sample size was 100. Total enumeration technique was used. Microbiological cultural report and Modified UTI basic data sheet were used for assessing the prevalence of UTI in patients and Interview schedule was used for assessing the knowledge and practices of the patients and their caregiver regarding the prevention of UTI. The study was ethically approved by the Institution Ethics Committee of the hospital.Result: The mean age of the patients was 40 ±14.09 (in years) and mean age of caregivers was 36 ±13.55 (in years). Out of the total subjects most of them were married and male. The main cause of injuries was fall. About (10%) of patients had UTI. Subjects had more knowledge about sign and symptoms of UTI. Conclusion: Subjects had adequate knowledge about UTI but they need education and training programmes to improve practices to prevent it. Keywords: Knowledge, Practices, Spinal cord injury (SCI), Urinary tract infection (UTI)