Background: Vitamin D plays a crucial role in bone maintenance and immunity; there can be a biologic rationale to suspect that Vitamin D deficiency could negatively affect the periodontium. Material and method: 100 patients were enrolled in this study which was divided into two groups of 50 each: Group 1: 50 patients with clinical and radiographic diagnosis of chronic periodontitis, Group 2: 50 clinically healthy. All demographic data of these patients was obtained. Blood samples of the patients were collected. These samples were sent to laboratory for biochemical analysis of serum vitamin D levels. SPSS software was used for statistical analysis. Results: Out of 100 patients 33 patients were less than 40 years old and 67 patients were above 40 years of age. Males were more affected in the periodontal health with 30 out of 50 patients in the periodontitis group being males. It was observed in this study that the rural population was more affected with periodontitis with 33 out of 50 patients having a rural background. Mean serum Vitamin D levels among the patients of the periodontitis group were found to be 20.31ng/ ml. Mean serum Vitamin D levels among the subjects of the control group were found to be 21.49ng/ml. The standard deviation of the periodontitis group and the control group came out to be ±11.52 and ±10.87 respectively. Conclusion: There was little difference between the serum Vitamin D levels of healthy subjects and patients with chronic periodontitis. Key words: Vitamin D, chronic periodontitis