Background: It is well studied that obesity is linked with decreased serum testosterone levels and obese men are found to have increased estrogen levels, thus it can be assumed that testosterone to estradiol ratio in obese men should be less as compared to individuals with normal BMI. Hence; under the light of above mentioned data, the present study was undertaken for assessing the testosterone levels in obese non-obese patients. Materials & methods: A total of 25 obese patients and 25 non-obese patients were enrolled in the present study. Five millilitre of venous blood was collected in fasting state, drawn into plain and EDTA vacutainers, and then centrifuged and serum separated. The sera were used to measure the concentration of Testosterone by Chemiluminescence. All the results were recorded in Microsoft excel sheet and were analysed by SPSS software. Student t test was used for assessment of level of significance. Results: Mean testosterone levels among the subjects of obese group and non-obese group was found to be 7642.81 picomol/L and 22341.85 picomol/L respectively. While comparing the mean testosterone levels among patients of both the study groups, significant results were obtained. Conclusion: Testosterone levels are significant correlated with BMI. Obese patients are associated with significantly lower testosterone levels. Key words: Testosterone, Obesity