ABSTRACT Background: Intestinal parasitic infections have always been an important medical and public health issue in tropics, especially in developing countries like India. The present study was conducted to assess intestinal parasitic infection among patients visited in tertiary care hospital. Materials & Methods: The present study was conducted on 126 cases of parasitic infection which included 70 males and 56 females. Stool samples in all patients were collected in sterile glass container. The stool specimens were routinely examined using direct wet mount (saline) and iodine wet mount preparation to detect protozoal tropozoites, helminth eggs or larvae and parasitic cysts by microbiologist. Results: Age group 21-30 years had 12 males and 10 females, 31-40 years had 20 males and 12 females and 41-50 years had 16 males and 18 females, >50 years had 22 males and 16 females. Common parasitic infection was E. coli (40), E. histolytica (32), T. Hominis (26), Giardia lamblia (14), blastocystitis (8) and taenia species (6). The difference was significant (P< 0.05). Pattern of infection was single (72), double (36) and triple (18). The difference was significant (P< 0.05). Conclusion: Maximum cases were observed in males. Common infection was E. coli followed by E. histolytica.