Introduction:- Pulmonary tuberculosis is a disease of the respiratory system, caused by Mycobacterium tuberculosis. Tuberculosis is a predominant infectious cause of mortality today. According to World Health Organization (WHO), tubercular infections are currently spreading at the rate of one person per second per million people. The study of sensitivity of Fluorescence staining versus Ziehl-Neelsen fuchsin staining plays an important role in diagnosing sputum smear positive pulmonary tuberculosis. Materials And Methods:-500 sputum samples collected from 250 suspected cases of pulmonary Tuberculosis attending the microbiology and immunology department between December 2016 to December2017 were processed and subjected to ZN and FM staining for detection of AFB. Positive smears were graded according to standard RNTCP criteria. Results:-Out of 500 sputum smears, 12% and 19.8% were positive by ZN and FM staining respectively. FM staining was found to be superior to ZN staining in detecting TB cases and also FM staining was able to detect more paucibacillary cases than ZN. Conclusion:- Our study concludes that FM staining with LED is more efficient over ZN stain in detecting Tuberculosis bacilli in sputum, especially the paucibacillary cases. Keywords – FM staining, ZN staining, Paucibacillary Tuberculosis