Background: Laparoscopic cholecystectomy (LC) has become the gold standard for surgical Abstract treatment of benign gallbladder disease. The advantages of this procedure compared to laparotomy include smaller incisions, reduced surgical trauma, less postoperative pain, faster recovery times, a shorter hospital stay, and faster return to normal activities and work. Hence; we planned the present study to assess the serum lipid profile in patients undergoing LC. Materials & methods: The present study included assessment of serum lipid profile of patients undergoing LC. Blood samples were taken after a 12 hour fasting state at the baseline and completion of the study and were kept in -70 oC until they were assessed. Serum concentrations of triglyceride (TG), total cholesterol (TC), high- density lipoprotein cholesterol (HDL-C), were measured before and 30 days after the surgery by using specific kit and the serum concentration of low-density lipoprotein cholesterol (LDL-C) were calculated. All the results were recorded on excel sheet and were analyzed by SPSS software. Results: Non- significant results were obtained while comparing the mean serum lipid profile in patients undergoing LC.Conclusion: Serum lipid profile appears to be unaffected by LC procedure. Key words: Cholecystectomy, Laparoscopic, Lipid